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产品目录

MalI内切酶图片
产品货号:
GS2225
中文名称:
MalI内切酶
英文名称:
MalI
产品规格:
250U
发货周期:
1~3天
产品价格:
询价
储存条件:冷冻(-20℃)

使用说明

To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?

技术规格

Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sitesA↑CCWGGT
来源Microbacterium arborescens SE
Assayed onLambda DNA
Optimal bufferW(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.)+ BSA
Optimal temperature37℃
Storage conditions10mMTris-HCl(pH 7.5);100 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA,50%glycerol;Store at -20℃.
LigationsAfter 5-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃.
Methlation effectNot blocked by overlapping Dcm methylation(CmCWGG)ACCWGGT.
Thermal inactivationenzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible EndsBfuCI^,BscFI^,Bsp143I^,BssMI^,BstENII^,BstKTI^,BstMBI^,DpnI^,Kzo9I^,MboI^,NdeII^,Sau3AI^

相关搜索:MalI内切酶MalI限制性内切酶
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